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In short, M9 minimal medium with 24 g L 1 glycerol as the carbon source and supplemented antibiotics as stated above and 0.1 mM Isopropyl--D-thiogalactopyranosid (IPTG) was used for the cultivation at 37C in 48-well microtiter FlowerPlates with the Biolector cultivation system (m2p-laboratories GmbH, Baesweiler, Germany) (Kensy et al., 2009)

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A lack of vitamins slows down energy production and your body then uses the available muscle proteins

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