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Endoplasmic reticulum (ER) stress is a secondary response caused by oxidative stress or inflammation mediated by aggregated proteins 14,15

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The buffy coat was resuspended in Roswell Park Memorial Institute (RPMI)-medium enriched with 5% fetal bovine serum, 1 M HEPES buffer, 2 mM L-glutamine, 5 mM -mercaptoethanol, 10,000 U/ml penicillin and 10 mg/ml streptomycin solution

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Medications like ranitidine and finasteride, as FMO substrates, competitively inhibit TMA binding, consequently diminishing TMAO generation and potentially mitigating CKD progression