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Making Liposomal Vitamin C Practical Guide Adapted from Materials Scale Blender Ultrasonic bath (if you don't have one, see instructions for alternative) Thermometre (if you don't have an ultrasonic bath or you do and it doesn't have one built in) Glass beaker or container fitting in the ultrasonic bath 98% Ethyl alcohol Vitamin C (such as ascorbic acid) Lecithin granules Instructions Measure out the amounts of ingredients needed for 1 litre: 477.6 grams of Water 114.8 grams of ethyl alcohol 160.5 grams of vitamin C 197.1 grams of lecithin granules Lecithin Add the water, alcohol and vitamin C into the beaker Place the beaker into the ultrasonic bath* (not set to sweep) at 35C while irradiating, making sure it doesn't touch the sides or bottom Mix regularly until vitamin C is dissolved, about 15 minutes * If you dont have an ultrasonic bath, heat water, alcohol and vitamin C in a pot on the stove, while stirring
Motion beam detectors To quantify spontaneous locomotor activity, experimental mice and control littermates were placed in individual automated photocell activity cages (29 50 cm) with twelve 2 cm high infrared beam detectors in a 4 8 grid (San Diego Instruments, San Diego, CA)

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