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To explore the impact of ArfGAP1 phosphorylation on this Arf1-dependent activity, we evaluated Golgi morphology in human SH-SY5Y neural cells overexpressing ArfGAP1 phospho-site variants by substituting either uncharged hydrophobic phospho-null (Alanine: A) or negatively-charged phospho-mimicking (Aspartic Acid: D) residues at Ser284, T291 and T292

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Antioxidant defenses and glutathione The reality of oxidative stress is demonstrated by the existence, in all aerobic organisms, of several antioxidant enzymes devoted to ROS detoxification, such as peroxidase, catalase, superoxide dismutase, and peroxiredoxins

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Tissue homogenates were centrifuged at 12,000 rpm at 4 C for 20 min and the supernatant was collected