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Enzymatic reduction of disulf ide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT) Proc Natl Acad Sci U S A

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Membranes were stripped in Restore Plus Western Blot stripping buffer (46430, Thermo Fisher Scientific) for 1 h, washed with TBST (three times, 15 min each), blocked with 5% BSA and reprobed with primary antibody to eIF4E (Cell Signaling Technology) overnight at 4 C

N6-methyladenosine (m 6 A) demethylase FTO is significantly upregulated in MPP + -stimulated SK-N-SH cells, which suppresses YTH domain family protein 2 (YTHDF2) expression, promotes m 6 A demethylation of BRCA1-associated protein 1 (BAP1) and enhances BAP1 expression, upregulates p53 expression, and inhibits SLC7A11 transcription, thereby increasing MDA, Fe 2+ , and 4-HNE levels and triggering neuronal ferroptosis

Further studies are required to identify free radicals and their intermediates, evaluate evidence of radical attack on molecules, and estimate antioxidant status in hypertension

On the basis of the results available up to date, the Global Initiative for Chronic Obstructive Lung Disease [119] recognizes that mucolytic and antioxidant drugs, such as NAC, can be used as an adjunct therapy to reduce the risk of acute exacerbation of COPD, but in a limited way due to the lack of robust randomized controlled clinical trials (level of evidence B)