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Supernatants were isolated to quantify protein and 510 g protein samples were mixed with a 4X non-reducing buffer prior to separation by SDS-PAGE as described
doi: 10.1046/j.1365-201X.1997.576351000.x
This reaction forms NADH, which can be used as a reducing agent to donate electrons
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NAD levels decline significantly with age, affecting metabolism and cellular function
Cell 1 , 161170 ( 1998)
