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Vitamin C is a critical cofactor for collagen synthesis

Mar 2024;17(1):134-144

In their study, high-dose anti-VEGFR2 treatment aggravated intratumoral hypoxia and restrained the infiltration of CD8 + T cells into the TME, thereby suppressing anti-cancer immunity

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The tissue was disrupted using tissue homogenizer Bio-Gen PRO200 homogenizer (PRO Scientific, Oxford, CT, USA), in 1000 l of TRIzol (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and the isolation itself was done using a TRIzol-based reagent method according to the manufacturers instructions