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During the rapid weight loss phase, your body mobilizes both adipose tissue and lean mass

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Quantification of steroidogenic acute regulatory gene expression RNA Isolation, cDNA synthesis, and real-time quantitative polymerase chain reaction The homogenate was directly suspended in TRIzol reagent, followed by the extraction of mRNA

Separation of lipids was achieved on a Phenomenex Luna 3-m silica column (100- LC column, 150*2 mm) using a gradient from solvent A to solvent B (isopropanol/hexane/100 mM ammonium acetate 50/40/10

doi: 10.1038/nrmicro1789 10 DummlerA.LawrenceA

