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Loss of NAD + levels results in impairment of mitochondria function, which leads to failure of essential metabolic processes (Reid et al., 2018)

The hydroxyl radical scavenging activity (Y 2 ) was calculated by the following formula: where A s was the absorbance of the reaction solution with sample, A 0 was the absorbance of reaction solution with sample replaced by equal volume of deionised water, A c was the absorbance of the reaction solution with sample and hydrogen peroxide replaced by equal volume of deionised water

Another promising method is the supercritical carbon dioxide (CO 2 )-based decellularization, which may better preserve the native composition of the ECM (Matthews et al., 2018)

By scavenging excessive ROS, polysaccharides can prevent the activation of pro-tumorigenic pathways (e.g., NF-B and MAPK) that are driven by oxidative stress (136)