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Corresponding Author: Mohsan Ali, MBBS, King Edward Medical University, St 12, Wapda Town, Phase #1, Lahore, Pakistan ([email protected])

Unlisted potential side effects include, but are not limited to, vision loss, stomach paralysis and intestinal blockages

Tirzepatide is a novel agent that acts as an agonist for both GLP-1 receptors and gastric inhibitory polypeptide (GIP) receptors, another incretin that lowers glucose and appetite

The presence of GLP-1 receptors in brain regions central to reward processing (Lpez-Ojeda & Hurley, 2024), together with evidence that GLP-1 RAs can modulate activity within these circuits (Gabery et al., 2020), has led to the hypothesis that GLP-1 RAs may reduce vulnerability to multiple maladaptive reward-seeking behaviors by blunting neuroaffective responses to reward-related cues (Eren-Yazicioglu et al., 2021

Sample sections were blocked in 5 % goat serum with 0.3 % Triton X-100 for 1 h at RT and then incubated overnight at 4 C with the following primary antibodies: mouse anti-GLP-1R (1:50, Santa Cruz, CA, USA), mouse anti-GLP-1(1:50, Santa Cruz, CA, USA), rabbit anti-Iba1 (1:500, Wako Chemicals, Tokyo, Japan), rabbit anti-GFAP (1:200, Cell Signalling, Boston, MA, USA), rabbit anti-NeuN (1:100, Proteintech, China), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), and mouse anti-c-fos (1:100, Santa Cruz, CA, USA)

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