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The two samples were sent to Biomarker Technologies (Beijing, China) for High throughput sequencing (HTS) RNA-Seq sequencing after depletion of the rRNAs with Epicentre Ribo-ZeroTM kit, which was then sequenced using the Illumina HiSeq X-ten platform with PE150 bp (Illumina, San Diego, CA, USA)

Each cartridge contains 1.2 ml of e liquid and delivers up to 400 puffs per cartridge

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Tobacco smoking is the leading cause of chronic obstructive pulmonary disease (COPD), a condition where the build-up of pus-filled mucus in the lungs results in a painful cough and agonizing breathing difficulties

The urine collected for this study was to be only tested for cotinine and no other substances