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First: PI3K/Akt/mTOR, which drives protein synthesis, glucose uptake (via GLUT4 translocation), and anti-apoptotic signaling (particularly relevant in muscle and neuronal tissue)

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Washing steps remove weakly or non-specifically bound proteins without disrupting the specific bait-prey complexes

Their team provided mass spectrometry confirmation and troubleshooting support, which was crucial for moving the project to structural analysis. Head of Structural Biology | Pharmaceutical Research Lab Pull-Down Assays Service: Frequently Asked Questions (FAQs) Q: What types of proteins can be analyzed with pull-down assays