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Inhibition of Dll4-mediated signaling induces proliferation of immature vessels and results in poor tissue perfusion

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TEM The cells were fixed overnight in a mixture of cold 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.2) and 2% paraformaldehyde in 0.1 M phosphate or cacodylate buffer (pH 7.2)