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Reconstitute only what youll use within 28 days for longer studies, store additional vials as lyophilised powder at 20C and reconstitute serially

Samples were stored at 20 C for 10 min and centrifuged (2,000 g, 15 min, room temperature)

(2) Meridians and collaterals, which are the pathways for qi and blood to flow throughout the human body, form a comprehensive network linking the internal organs and tissues into an organic whole [Figure 3]

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Affinity purifications and immunoprecipitation/pull-down experiments both have the same discrete bind, wash, and elute steps (Figure 3)