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In rodents, inhibiting the glutamate-cysteine ligase enzyme depleted the brain GSH, inducing cognitive symptoms like that observed in schizophrenia (Gysin et al

Shift the temperature to 30 C and induce the culture(s) with IPTG at a final concentration of 1 mM (5 mL of 200 mM IPTG stock solution per liter of culture)

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