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Nickel affinity chromatography The nickel on agarose beads binds to proteins through polyhistidine tags, known as his-tags, that usually range from 6 to 10 histidine residues long (Figure 2)

Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli

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