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Beads were subsequently washed with 10 column volumes (cv) of 1x150 mM NaCl, 5 mM EDTA in 50 mM Tris, 1x150 mM NaCl in 50 mM Tris, 1x450 mM NaCl in 50 mM Tris and 50 mM Tris buffer without salt

The mRNA levels of CCL2 (C-C Motif Chemokine Ligand 2), CCL11 (C-C Motif Chemokine Ligand 11), CSF2 (Colony Stimulating Factor 2), CXCL10 (C-X-C Motif Chemokine Ligand 10), HGF (Hepatocyte Growth Factor), IL1RA (Interleukin 1 Receptor Antagonist), IL6 (Interleukin 6), IL10 (Interleukin 10), IL31 (Interleukin 31), LIF (LIF Interleukin 6 Family Cytokine), and NGF (Nerve Growth Factor) were quantified using specific primers in SYBR Green (Thermo Fisher) or TaqMan (Thermo Fisher) platforms following the manufacturers instructions