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For LC separation, tryptic peptides were sequentially injected into an Acclaim PepMap 100 C18 column (100 M 2 cm, 5 M, Thermo Fisher Scientific) and an Acclaim PepMap 100 C18 column (50 M 15 cm, 2 M, Thermo Fisher Scientific)

GHK-Cu can upregulate genes linked to collagen and elastin