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After repeated washing the bound fraction was eluted with 250 mM imidazole (Fischer) in lysis buffer containing 10% glycerol (Acros)

Coppola, G

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ATF4 is a 351-amino acid cAMP-response element binding protein that belongs to the CREB-2 family of proteins

Meftahi et al., 2021)

According to our study, NAC could serve as an efficacious agent for suppressing ferroptosis-induced cytokine secretion