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If you are dosing 0.25 mg weekly, a 5mg vial provides 20 doses, which is still far more than the 4 doses you can use within 28 days

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Use multiple angiogenesis-associated endpoints: VEGFR2 expression, VEGFR2 phosphorylation, endothelial tube length, branch points, meshes, and cell viability each measure different aspects of endothelial response