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To 0.5 ml of the supernatant, 0.5 ml 0.5 M phosphate buffer (pH 7.8) and 0.05 ml Ellmans reagent (5 mM) were added

Why B12 Matters B12 deficiency becomes more common with age due to decreased absorption Many adults do not get enough B12 from diet alone Certain conditions (e.g., anemia, fatigue) may require supplementation Oral supplements are not always reliably absorbed Contact Details 2764 Aborn Road, San Jose, CA, USA

doi: 10.3389/fphar.2025.1643146 Received 18 June 2025 Revised 16 October 2025 Accepted 28 October 2025 Published 11 November 2025 Volume 16 - 2025 Edited by Karl Tsim, Hong Kong University of Science and Technology, Hong Kong, SAR China Reviewed by Qianghua Quan, Tsinghua University, China Jasmine Millman, Swisse Wellness Pty Ltd., Australia Updates Copyright 2025 Song, Sun, Yuan, Tang and Zheng

Therapeutic proteins would be released in the interstitial space [14] or intracellularly [20] by specific protease sites engineered between the GST and the therapeutic protein

In contrast, TCM operates through multi-target mechanisms that promote immune recalibration, support tissue repair, and facilitate holistic functional recovery

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