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The tissue samples were lysed in 1 x lysis buffer (20 mM Tris-HCL [pH 7.5], 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM Na3VO4, 1 mg/mL leupeptin), supplemented with protease inhibitor 1 mM PMSF

Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T, et al

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