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Reduced membrane fluidity in cells depleted of UFAs or BCFAs To confirm that changes in fatty acid composition translate to shifts in in vivo membrane fluidity, we monitored changes in steadystate fluorescence anisotropy of 1,6diphenyl1,3,5hexatriene (DPH), the rotational freedom of which is sensitive to acyl chain disorder and, thus, indirectly to the fluidity of lipid bilayers (Lentz, 1993)

Cells were cultured in a humified 5% CO 2 incubator at 37 C

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