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24 h after the treatment, cells were fixed with 10% trichloroacetic acid, extensively washed, then incubated with the dye, washed and finally resolubilized in 10 mM Tris base, prior to scanning the plates at 565 and 690 nm (main signal and background, respectively)

[DOI] [PMC free article] [PubMed] [Google Scholar] 35.Elliott-Sale K.J., Minahan C.L., de Jonge X.A.K.J., Ackerman K.E., Sipil S., Constantini N.W., Lebrun C.M., Hackney A.C

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Acknowledgements We would like to thank Veronika Gurianova and Anna Brattseva for their kind support

Only a limited number of animal studies have employed other methods to explore the possible benefits of ozone in AD