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This subpopulation of LARS B cells shows a preference for Leu metabolism, which can be induced by a Leu diet to promote mitochondrial NAD + production and oxidative metabolism and recruit NAD + -dependent protein deacetylase SIRTUIN-1 (SIRT1) to participate in the regulation of LARS B cells

The GCL enzyme is a heterodimer formed by two subunits: the heavy subunit or glutamate cysteine ligase catalytic subunit (GCLC, 73 kDa) and the light subunit or glutamate cysteine ligase modulating subunit (GCLM, 30 kDa)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 55.Richie JP, Nichenametla S, Neidig W, Calcagnotto A, Haley JS, Schell TD, Muscat JE

ADH and ALDH activities were measured using the ADH assay kit (#ab102533, Abcam, Cambridge, UK) and the ALDH activity assay kit (#ab155893, Abcam), respectively

In the cocaine-dependent patients, glutamate levels were normalized by a single oral administration of NAC (2.4 g) [128]