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Antibiotic use can have negative effects on the microbiome, such as reduced species diversity and altered metabolic activity

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cerevisiae and evaluate effect of promoter strength on its activity, four DNA-mediated integrative expression vectors pGAPg-gshF, pGAPg-gshF, pGAL1g-gshF and pPGK1g-gshF were constructed, in which the gshF gene was driven by glyceraldehyde-3-phosphate dehydrogenase promoter (GAP) of S

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The mixture underwent incubation in the dark for 10 min, and absorbances were determined at a wavelength of 734 nm

[DOI] [PMC free article] [PubMed] [Google Scholar] 173.Obata F., Miura M