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Competitive cell proliferation assays To assess the effect of CRISPR/ Cas9 -mediated gene targeting on cell proliferation, Cas9 -expressing cells were transduced with sgRNA/IRFP670 expression vectors and IRFP670-positive cells were monitored at regular intervals by flow cytometry using an IntelliCyt IQueScreener Plus (BioScience, Sartorius Group, Germany)
doi: 10.1136/gut.45.2.199

Furthermore, AT 1 R is involved in the progression of cardiovascular diseases including hypertension, atherosclerosis, cardiac hypertrophy, and heart failure (Stegbauer and Coffman, 2011

It has been effectively used to treat a variety of skin conditions, including acne, eczema, psoriasis, and rosacea

Astragaloside IV ameliorates isoprenaline-induced cardiac fibrosis in mice via modulating gut microbiota and fecal metabolites

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