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When under oxidative stress (OS) conditions, Hb is oxidized, it binds to the cytoplasmic domain of membrane protein band 3, triggering the formation of aggregates and the covalent linkage of natural anti-band 3 antibodies that may lead to premature RBC removal by splenic macrophages [1, 9]

Krus KL, et al

Likewise, the mRNA of inflammatory mediators (iNOS, IL-6, IFN and TNF-) are inhibited, with increased expression of HSP 70 and 90, and antioxidant proteins, such as HO-1, NQO-1, glutathione reductase, glutathione peroxidase 2 and GST-pi [19]

FIGURE 1 Samples collection and storage To confirm APAP toxicity, 0.3 mL of blood were collected from the tail vein 4 hours after administering the APAP dose

The deficiency of some vitamins such as folate, vitamin B12, and vitamin B6 increases the serum and tissue levels of homocysteine

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