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Plants were grown for 3 days until the characteristic dwarf phenotype of rml1 became visible

Subsequently, sections were incubated with ProtK (10 g/ml) for 5 min and washed twice with PBS-0.1% Tween

When this happens alongside a steep decline in NAD+, cells lose both the engine that powers repair and the shield that blocks damage

Suppression of early oxidative stress In our study, early increases in SOD, CAT, and GSH levels, along with decreased MDA levels, indicate that anakinra can effectively control reperfusion-induced oxidative damage during the early phase

doi: 10.1038/s41590-021-00993-3