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The cellular assays, including reactive oxygen species (ROS) assay (4 h-treatment), thiobarbituric acid reactive substances (TBARS) assay (6 h-treatment), glutathione (GSH) assay (6 h-treatment), and cell viability assay (12 h-treatment), were done as previously described [28] using a fluorescence spectrometer (VersaFluor, Bio-Rad Laboratories)

Administration methods and dosing protocols Different routes affect ocular tissue exposure and practical feasibility

However, individuals with absorption disorders may require significantly higher doses to achieve therapeutic levels

2 , e112 (2021)

Uptake of lycopene and its geometrical isomers is greater from heat- processed than from unprocessed tomato juice in humans

This hypoxic environment was maintained using a gas mixture (1% O 2 , 5% CO 2 , and 94% N 2 )