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They were washed three times with PBS for 5 min

We've written more about this in our [copper peptides vs retinol blog]

One possible explanation is that low serum vitamin B12 levels would trap folate as 5-methyltetrahydrofolate, prevent the generation of methionine from homocysteine, and therefore reduce protein synthesis and lean tissue deposition

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Youre Constantly Tired Even After a Full Nights Sleep This is the most common sign of low B12
Low levels can make it hard to concentrate or stay engaged
