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Cells were cultured for 24 hours in the presence of CHIR99021, followed by 24 hours in either RPMI/B27 with or without insulin

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Oral Dosing Runs Higher Injectable: 200 to 500mcg per dose Oral: 500 to 1,000mcg per dose, once or twice daily, taken on an empty stomach Higher oral doses are used to make up for the fact that the gut absorbs the compound poorly compared to injections

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