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After confirmation of protein transfer using Ponceau S red staining, membranes were blocked in RotiBlock (Carl Roth GmbH & Co.KG) for 30 min

Gelpi E, Rahimi J, Klotz S, Schmid S, Ricken G, Forcen-vega S, et al

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D., Glover E

NOD-like receptors-pivotal guardians of the immunological integrity of barrier organs

Springer, Dordrecht, pp 397415