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The pentose phosphate pathway, galactose metabolism, and the metabolism of amino sugars and nucleotide sugars are observed to be active by the middle of fermentation

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Bacteria were harvested by centrifugation at 7,000 rpm 15 min and washed 10 times with a buffer containing (per liter) 8 g NaCl, 0.2 g KCl, and 0.24 g Na 2 HPO 4 (pH 7.4)