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The experimenter was blinded to the genotype of the mice during behavior testing and until the mice were end pointed

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The membranes were blocked with Starting Block T20 PBS Blocking Buffer (Thermo Fisher Scientific, Inc.) and probed overnight at 4C with the primary antibodies diluted with 10% Blocking Ace (DS Pharma Biomedical) in PBS containing 0.1% Tween-20

Tissue Antigens 66, 291296
Free Radic Biol Med 31(4):509519 Moors TE, Hoozemans JJM, Ingrassia A, Beccari T, Parnetti L, Chartier-Harlin MC, van de Berg WDJ (2017) Therapeutic Potential of Autophagy-Enhancing Agents in Parkinsons Disease

Transcriptional regulation of Nrf2 Binding of Aryl hydrocarbon receptor-aryl hydrocarbon receptor nuclear translocator (AhR-ARNT) to xenobiotic response element (XRE) sequences is known to regulate Nrf2 activation
