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This process is kinetically slow under biological conditions of neutral pH, due to the very low concentration of the free thiolate groups, which are the true active agents of the reaction, as depicted in Scheme 12

Without enough of these proteins, fluid can leak out and cause swelling

Tesniere A, Schlemmer F, Boige V, Kepp O, Martins I, Ghiringhelli F et al

10.1038/s41568-021-00417-2 [DOI] [PMC free article] [PubMed] [Google Scholar] Geissler R, Simkin A, Floss D, Patel R, Fogarty EA, Scheller J, & Grimson A (2016)

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Increased DNMT1 acetylation leads to global DNA methylation suppression in follicular granulosa cells during reproductive aging in mammals