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As previously noted, ursodeoxycholic acid (or Ursodiol) is currently used to treat gallstone disease in patients for whom surgery is not an option, but widening its use has been proposed, such as to reduce the risk of gallstone formation during pregnancy or rapid weight loss, after abdominal surgery, and in infants requiring long-term total parenteral nutrition (Portincasa 2012

Desalt the supernatant with PD-10 columns equilibrated with 100 mM NaCl containing 20 mM Na + /K + phosphate buffer pH 7.4 (or 20 mM Tris base for rat RBCs)

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Additionally, the experiment did not include recruitment of disease controls, so the specificity of the technique to ME/CFS is unknown