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The cells were then lysed, and the lysates were cleared by centrifugation at 33,745 x g for 30 min at 4 C

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The authors reported that levels of reduced glutathione GSH were significantly reduced and the levels of oxidized glutathione GSSG were significantly increased in the cerebellum and temporal cortex in the brain samples from the group with autism as compared to the corresponding levels in the control brain samples
