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Total RNA was extracted in triplicate as described, and cDNA was synthesized with the iScript reverse-transcriptase kit (Bio-Rad, Hercules, CA)

Peenik M, et al

Would this be some kind of rebound effect, like an immune (or autoimmune) reaction to the injection/B12

The anatomical basis for the immune function of the gut

It outlines their influence on beneficial microorganisms, such as Bifidobacterium , Prevotella , Lactobacillus , and Faecalibacterium prausnitzii , alongside potentially pathogenic bacteria, including Bacteroides fragilis , Salmonella Typhimurium , and Campylobacter jejuni