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The cell line was maintained in DMEM supplemented with 10% FBS, 200 mM Ala-glutamine (Sigma Aldrich), and 50 U/mL Gentamicin (Life Technologies), and cultured at 37 C with 5% CO2 incubation

NAD+ plays a crucial role in regulating cellular and metabolic signaling pathways , which is a fancy way of saying it helps coordinate everything from how your body burns calories to how it repairs damaged DNA

Casarin, V., Plassard, C., Souche, G

doi: 10.1007/s11064-016-2018-6

In the reduced state, the thiol group of cysteinyl residue is a source of one reducing equivalent

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