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Then samples were immediately frozen on dry ice/ethanol and thawed on running tap water
Next, dimensionality reduction and derivation of variable importance in projection (VIP) values were performed through orthogonal partial least squares-discriminant analysis (OPLSDA) implemented with the R package Ropls

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Arachidonic acid (AA) and linoleic acid (LA) are used as substrate of lipoxygenases (LOXs) which catalyses di-oxygenation of esterified as well as free PUFAs delivering several lipid hydroperoxides [86, 87]
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