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These types of calculations often use alchemical free energy methods to estimate phase transfer via a non-physical thermodynamic cycle

Gasch, A.P., Spellman, P.T., Kao, C.M., Carmel-Harel, O., Eisen, M.B., Storz, G., Botstein, D

Radiology 270 , 658679 (2014)
Instead, they represent potential tools for targeted activation of innate immune memory

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