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For analysis by SDS-PAGE and Western blotting, proteins were eluted (30 min, 50 C) in 2 NuPAGE LDS sample buffer (Thermo Fisher Scientific, NP0007) containing 50 mM DTT (Sigma-Aldrich, D0632)

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Plant Signal Behav 5:171175 Kelly GJ, Latzko E (1979) Soluble ascorbate peroxidase

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This validates the presence of functional GLY III enzyme in rice that can carry out MG detoxification following a shorter GSH-independent route in contrast to the conventional GSH-dependent two step glyoxalase I/II pathway