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When combined with training, KETO restored muscle remodeling processes that are blunted with hyperglycemia, including an increase in oxidative muscle fibers and capillary density

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Briefly, HEK 293 T cells (Thermo Scientificl #HCL4517) were transfected with the calcium phosphate method with an equimolar mixture of the expression plasmid, pHelper plasmid, and Rep/Cap plasmid pAAV2/DJ (for the production of the shRNA, pAAV2/1 and pAAV2/2 were combined to obtain a mixed capsid of AAV1/2)

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PMID: 34260042