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The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters

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Quantified fractions of target RNA bound across AGOmiRNA dilutions were fit to a quadratic equation by nonlinear least-squares regression in R using the LevenbergMarquardt algorithm (nlsLM from the R package minpack.lm): where F bound represents fraction of target bound, [target T ] represents the concentration of total target oligonucleotide, [stock] represents stock concentration of RISC, DF represents the dilution factor, K D represents the dissociation constant for the affinity between RISC and the target and F max represents the maximal fraction of target bound at the plateau

[DOI] [PMC free article] [PubMed] [Google Scholar] 73.Tardy A-L, Pouteau E, Marquez D, Yilmaz C, Scholey A