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Additionally, stress-inducing agents such as tunicamycin, thapsigargin and histidinol have been shown to modulate CHAC1 regulation, indicating its involvement not only during ER stress but also in the context of the ISR ( CHAC1 promoter is the primary mechanism for the transcriptional upregulation of the gene

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In terms of biochemical characteristics, cells undergoing ferroptosis show an imbalance of oxidationreduction levels: a significant increase in intracellular reactive oxygen species (ROS) and a significant decrease in NADPH, and therefore lipid antioxidants can inhibit ferroptosis