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10.1053/j.gastro.2013.04.056 90

Differential scanning fluorimetry (DSF) DSF was performed either by a dye-based method using a Lightcycler RT-PCR instrument (Roche) or label-free using a Prometheus NT.48 nanoDSF instrument (Nanotemper Technologies GmbH)

Enhanced Absorption with Liposome Technology

This compromises the relaxation of vascular smooth muscle cells and has a degenerative effect on the vessels causing tissue death [34] and favoring the development of microvascular complications of diabetes, such as diabetic nephropathy (DN) (Figure 4)

Sample preparation for label-free liquid chromatography mass spectrometry Liver homogenates were treated using the Ready Prep 2D clean up kit from BioRad Laboratories, and re-suspended in label-free solubilisation buffer (6 M urea, 2 M thiourea, 10 mM Tris, pH 8.0 in LCMS grade water)
[DOI] [PMC free article] [PubMed] [Google Scholar] 67.Tang G.-Y., Zhao C.-N., Xu X.-Y., Gan R.-Y., Cao S.-Y., Liu Q., Shang A., Mao Q.-Q., Li H.-B
