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Next, the apo-enzyme was eluted using GST elution buffer (50 mM Tris, 10 mM methylglutathione, pH 8) before relevant fractions were combined according to their A 280nm

It might be hypothesized that any insufficiencies in phase I or phase II metabolism (Figure 3), which may lead to acetaminophen-induced autism, would be prevented by co-administration of NAC