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Expression of recombinant, tag-less parkin WT human parkin was generated as an initially 6His-Smt3-tagged protein in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (New England Biolabs), as described [3, 24, 50, 53]

10.1172/JCI.INSIGHT.93936 [DOI] [PMC free article] [PubMed] [Google Scholar] Bai B., Chen H

[DOI] [PMC free article] [PubMed] [Google Scholar] 24.Zamyatnin AA

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Short-Term Side Effects For most healthy users, reported side effects are mild and tend to resolve with dosage adjustment or time

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