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Preparations were then diluted 10X with buffer (50 mM tris-HCL, 2 mM EDTA and 100 mM NaCl) and washed 4 times using Amicon 10 kDa cutoff filter units (0.5 ml) to remove excess GSSG

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Kinetic modeling calculation The permeation of AuNPs through gaps opened in the BBB was calculated by integrating Eqs

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